Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Round spermatids
NA
NA

Attributes by original data submitter

Sample

source_name
Germ cells
tissue
Testis
cell type
germ cells
cell type
Wild type
developmental stage
round spermatids
chip antibody
none (Input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-seq data, we constructed the sequencing libraries using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB #7370L). For ChIP-seq data, we constructed the sequencing libraries using Next Gen DNA Library Kit and Next Gen Indexing Kit(ACTIVE MOTIF #53216&53264).

Sequencing Platform

instrument_model
HiSeq X Ten

mm10

Number of total reads
26804858
Reads aligned (%)
94.6
Duplicates removed (%)
5.7
Number of peaks
149 (qval < 1E-05)

mm9

Number of total reads
26804858
Reads aligned (%)
94.5
Duplicates removed (%)
5.7
Number of peaks
112 (qval < 1E-05)

Base call quality data from DBCLS SRA