Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Blood
Cell type
SUP-B15
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
SUP-B15
treatment
dexamethasone
cell line
SUP-B15
cell type
B-cell acute lymphoblastic leukemia line
translocation
t(9;22)(q34;11) → BCR-ABL1 e1-a2 fusion
antibody
Glucocorticoid receptor N499

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cels were crosslinked with formaldehyde, quenched, sheared with a Covaris sonicator, and IP'd with an antibody specific for GR (N499, a gift from K. Yamamoto). Standard protocllusing NuGen Ovation low DNA kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
299941992
Reads aligned (%)
68.9
Duplicates removed (%)
60.4
Number of peaks
14225 (qval < 1E-05)

hg38

Number of total reads
299941992
Reads aligned (%)
71.2
Duplicates removed (%)
59.5
Number of peaks
16787 (qval < 1E-05)

Base call quality data from DBCLS SRA