Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Larvae
Cell type
Salivary glands

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
salivary glands
genotype
SuUR mutant
chip antibody
none
tissue
3rd instar larvae

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20 pairs of 3rd instar larvae salivary glands were used as starting material. Cell Signaling Technology #9733 anti-H3K27me3 or Millipore #07-452 anti-H3K27me2 antibodies were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using Illumina Nextera protocol.

Platform Information


instrument_model
Illumina MiSeq

External Database Query

Logs in read processing pipeline


Number of total reads
554338
Reads aligned (%)
92.6
Duplicates removed (%)
1.2
Number of peaks
67 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA