Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SKI

Cell type

Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
immortalized cell line
cell line
HL60
cell type
acute myeloid leukemia
genotype/variation
CRISPR/Cas9-mediated deletion of SKI
chip antibody
anti-SKI (sc-9140, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in the presence of 1% formaldehyde. Cell lysates were sonicated and Protein-DNA complexes were enriched by immunoprecipitation with mentioned antibodies. Libraries were prepared according to the manufacturer's protocol (Diagenode, MicroPlex Library Preparation Kit).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
20745377
Reads aligned (%)
94.7
Duplicates removed (%)
3.0
Number of peaks
817 (qval < 1E-05)

hg38

Number of total reads
20745377
Reads aligned (%)
96.8
Duplicates removed (%)
1.9
Number of peaks
648 (qval < 1E-05)

Base call quality data from DBCLS SRA