Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
immortalized cell line
cell line
HL60
cell type
acute myeloid leukemia
genotype/variation
WT
chip antibody
anti-RUNX1 (ab23980, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in the presence of 1% formaldehyde. Cell lysates were sonicated and Protein-DNA complexes were enriched by immunoprecipitation with mentioned antibodies. Libraries were prepared according to the manufacturer's protocol (Diagenode, MicroPlex Library Preparation Kit).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
20414702
Reads aligned (%)
88.4
Duplicates removed (%)
5.2
Number of peaks
6938 (qval < 1E-05)

hg38

Number of total reads
20414702
Reads aligned (%)
90.3
Duplicates removed (%)
4.1
Number of peaks
6870 (qval < 1E-05)

Base call quality data from DBCLS SRA