GSM2871152: HL60 CTR-1 H3K27ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
immortalized cell line
cell line
HL60
cell type
acute myeloid leukemia
genotype/variation
CRISPR/Cas9 control
chip antibody
anti-H3K27ac (39133, Active Motif)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in the presence of 1% formaldehyde. Cell lysates were sonicated and Protein-DNA complexes were enriched by immunoprecipitation with mentioned antibodies. Libraries were prepared according to the manufacturer's protocol (Diagenode, MicroPlex Library Preparation Kit).