Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
USP7

Cell type

Cell type Class
Blood
Cell type
CUTLL1
NA
NA

Attributes by original data submitter

Sample

source_name
CUTLL1
cell line
CUTLL1
cell type
human T cell leukemia cells
treatment
untreated
chip antibody
anti-USP7 (Bethyl, catalog# A300-033A, lot# Lot A300-033A-7)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
USP7 ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human USP7 (Bethyl, A300-033A). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
33574246
Reads aligned (%)
91.5
Duplicates removed (%)
6.7
Number of peaks
30019 (qval < 1E-05)

hg38

Number of total reads
33574246
Reads aligned (%)
92.7
Duplicates removed (%)
6.1
Number of peaks
30008 (qval < 1E-05)

Base call quality data from DBCLS SRA