Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cell
chip antibody
input
genotype
MLL3-/-;MLL4-/-
cell type
embryonic stem cell
genetic background
C57BL/6 and SV/129

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, suspended 10 x 106 ESCs (feeder removed) and EBs are fixed with 1% formaldehyde in culture medium, followed by ChIP assay. For RNA-Seq, total RNA are collected using Trizol extraction followed by mRNA purification using Dynabeads® mRNA DIRECT™ Kit (invitrogen). Libraries were prepared according to Illumina TruSeq RNA and DNA sample preparation Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
25770061
Reads aligned (%)
85.2
Duplicates removed (%)
7.3
Number of peaks
377 (qval < 1E-05)

mm9

Number of total reads
25770061
Reads aligned (%)
85.0
Duplicates removed (%)
7.3
Number of peaks
393 (qval < 1E-05)

Base call quality data from DBCLS SRA