GSM1220852: input in MLL3-/-;MLL4flox/flox ESCs; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse embryonic stem cell
chip antibody
input
genotype
MLL3-/-;MLL4flox/flox
cell type
embryonic stem cell
genetic background
C57BL/6 and SV/129
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, suspended 10 x 106 ESCs (feeder removed) and EBs are fixed with 1% formaldehyde in culture medium, followed by ChIP assay. For RNA-Seq, total RNA are collected using Trizol extraction followed by mRNA purification using Dynabeads® mRNA DIRECT™ Kit (invitrogen). Libraries were prepared according to Illumina TruSeq RNA and DNA sample preparation Kit