Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293
transfection
DAXX-Myc
chip antibody
Myc Tag, Abcam #ab9132
transgene
Stable and unique insertion of a 5xUAS followed by Luciferase sequence in the first intron of NCOA5 (Stielow et al. EMBO Rep. 2008)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 15 minutes before lysis and chromatin extraction. ChIP were performed overnight using Dynabeads (ThermoFisher Scientific) coupled with the appropriate antibodies. Beads were washed and crosslinking was reversed by heating samples at 65˚C overnight. Recovered DNA was subsequently purified with MinElute PCR Purification Kit (Qiagen) according to the manufacturer's protocol. ChIP-Seq_NEB ultraII according to manufacturer's instructions

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
27119906
Reads aligned (%)
181.5
Duplicates removed (%)
3.4
Number of peaks
640 (qval < 1E-05)

hg38

Number of total reads
27119906
Reads aligned (%)
183.2
Duplicates removed (%)
3.2
Number of peaks
920 (qval < 1E-05)

Base call quality data from DBCLS SRA