Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

SH-SY5Y

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

SH-SY5Y cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and IR-DNA or Pol2-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed using Thruplex DNaseq kit (Rubicon) according to manufacturer's instruction.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

51775349

Reads aligned (%)

96.7

Duplicates removed (%)

4.0

Number of peaks

1466 (qval < 1E-05)

hg19

Number of total reads

51775349

Reads aligned (%)

95.8

Duplicates removed (%)

5.3

Number of peaks

1284 (qval < 1E-05)