Antigen

Antigen Class

TFs and others

Antigen

INSR

Cell type

Cell type Class

Neural

Cell type

SH-SY5Y

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

SH-SY5Y cells

chip antibody

rabbit, anti-IR_beta, sc-711

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and IR-DNA or Pol2-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed using Thruplex DNaseq kit (Rubicon) according to manufacturer's instruction.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

45984792

Reads aligned (%)

97.8

Duplicates removed (%)

4.3

Number of peaks

2182 (qval < 1E-05)

hg19

Number of total reads

45984792

Reads aligned (%)

96.7

Duplicates removed (%)

6.0

Number of peaks

1857 (qval < 1E-05)