Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
INSR

Cell type

Cell type Class
Neural
Cell type
SH-SY5Y
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
SH-SY5Y cells
chip antibody
rabbit, anti-IR_beta, sc-711

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and IR-DNA or Pol2-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed using Thruplex DNaseq kit (Rubicon) according to manufacturer's instruction.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
45984792
Reads aligned (%)
90.9
Duplicates removed (%)
6.0
Number of peaks
1857 (qval < 1E-05)

hg38

Number of total reads
45984792
Reads aligned (%)
93.5
Duplicates removed (%)
4.3
Number of peaks
2182 (qval < 1E-05)

Base call quality data from DBCLS SRA