Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
INSR

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2 cells
chip antibody
rabbit, anti-IR_beta, sc-711

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and IR-DNA or Pol2-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed using Thruplex DNaseq kit (Rubicon) according to manufacturer's instruction.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
23598136
Reads aligned (%)
89.9
Duplicates removed (%)
4.1
Number of peaks
1640 (qval < 1E-05)

hg38

Number of total reads
23598136
Reads aligned (%)
91.9
Duplicates removed (%)
3.0
Number of peaks
1755 (qval < 1E-05)

Base call quality data from DBCLS SRA