Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA

Attributes by original data submitter

Sample

source_name
human dermal fibroblasts (HDFs)
chip antibody
endogenous AR antibody (Santa Cruz Biotechnology)
cell type
dermal fibroblast

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immune-precipitation was carried from 15 × 10^6 HDFs as for the ChIP assay with anti-AR antibody versus nonimmune IgGs. The bead-bound chromatin was tagged with Tn5 transposase (Nextera DNA Sample Prep kit, Illumina). The “tagmented” chromatin was de-cross-linked, purified, and amplified (20 cycles) using tag-specific primers. The amplified products were subjected to size selection (200-400 bp) and sequenced using Illumina Hi-seq.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
46614685
Reads aligned (%)
37.0
Duplicates removed (%)
13.2
Number of peaks
2707 (qval < 1E-05)

hg38

Number of total reads
46614685
Reads aligned (%)
38.5
Duplicates removed (%)
11.4
Number of peaks
3053 (qval < 1E-05)

Base call quality data from DBCLS SRA