Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Mast Cells
MeSH Description
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.

Attributes by original data submitter

Sample

source_name
peripheral blood-derived mast cells
cell type
peripheral blood-derived mast cells
passages
>98% FcεRI+ CD117+, 10 weeks
source
CD34+ derived
chip antibody
H3K27Ac (ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
CHIP-seq libraries were prepared with NEB NEXT Ultra kit using 10 ng starting material Single read - Illumina nextseq 500

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
36599450
Reads aligned (%)
98.6
Duplicates removed (%)
10.1
Number of peaks
37921 (qval < 1E-05)

hg19

Number of total reads
36599450
Reads aligned (%)
98.5
Duplicates removed (%)
10.1
Number of peaks
37878 (qval < 1E-05)

Base call quality data from DBCLS SRA