Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Myeloid Cells
MeSH Description
The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).

Attributes by original data submitter

Sample

source_name
Myeloid precursors
strain
C57BLACK6
age
4-6 weeks
cell type
Bone marrow myeloid cells
treated with
CSF1 only
chip antibody
Non specific IgG (control) PreImmune Affinity purified.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated soluble chromatin was immunoprecipitated with 5 micrograms of PU.1 or MITF antibodies and pulled down by protein G sepharose beads equillibriated with yeast tRNA and BSA. Library construction was perfomed by modified Illumina ChIPseq library preparation kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
16506106
Reads aligned (%)
51.6
Duplicates removed (%)
37.8
Number of peaks
748 (qval < 1E-05)

mm9

Number of total reads
16506106
Reads aligned (%)
51.4
Duplicates removed (%)
38.1
Number of peaks
846 (qval < 1E-05)

Base call quality data from DBCLS SRA