GSM1219708: input DNA GFP D5 myocytes; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adipocyte
Cell type
Brown preadipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
MyoD-induced mouse brown preadipocytes, WT, input
strain/background
C57BL/6J
genotype
MLL3-/-
age
E18.5 embryos
cell type
MyoD-induced immortalized brown preadipocytes
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with antibody (MyoD: Santa Cruz Biotechnology, sc-760 (lot: H2107); H3K4me1: abcam, ab8895 (lot: GR61280-1); H3K4me3: Millipore, 17-614 (lot: 2109590); H3K27ac: abcam, ab4729 (lot: GR81163-1); homemade MLL4). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.