GSM1219532: Input DNA C/EBPbeta Cre; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adipocyte
Cell type
Brown preadipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
mouse brown preadipocytes, MLL4 KO, C/EBPbeta Cre, input
strain/background
C57BL/6J
genotype/variation
MLL3-/-;MLL4-/-
age
E18.5 embryos
cell type
immortalized brown preadipocytes
overexpression
C/EBPbeta Cre
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with antibody (C/EBPb: Santa Cruz Biotechnology, sc-150X (lot: L2910); H3K4me1: abcam, ab8895 (lot: GR61280-1); H3K27ac: abcam, ab4729 (lot: GR81163-1); homemade MLL4). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.