Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ebf1

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
EBF1_ChIP_6hrs
cell type
Ebf1-deficient B cell progenitors
stage post dox treatment
6 hrs
antibody
BioGenes 1C

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 minutes. Then they were washed with PBS and lysed for sonication. Chromatin was precipitated with a specific antibody (anti-EBF1, BioGenes, 4µg/sample) and protein A or G-coupled sepharose beads. After elution from the beads, chromatin was decrosslinked and purified using MinElute PCR Purification Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared via the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® library preparation protocol according to manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
56996459
Reads aligned (%)
97.9
Duplicates removed (%)
11.1
Number of peaks
7022 (qval < 1E-05)

mm9

Number of total reads
56996459
Reads aligned (%)
97.7
Duplicates removed (%)
11.9
Number of peaks
7022 (qval < 1E-05)

Base call quality data from DBCLS SRA