Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
input_proB
cell type
Ebf1-deficient B cell progenitors
stage post 4-oht treatment
CD19+
antibody
n.a.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 minutes. Then they were washed with PBS and lysed for sonication. Chromatin was precipitated with a specific antibody (anti-EBF1, BioGenes, 4µg/sample) and protein A or G-coupled sepharose beads. After elution from the beads, chromatin was decrosslinked and purified using MinElute PCR Purification Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared via the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® library preparation protocol according to manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
38123189
Reads aligned (%)
98.3
Duplicates removed (%)
12.1
Number of peaks
205 (qval < 1E-05)

mm9

Number of total reads
38123189
Reads aligned (%)
98.1
Duplicates removed (%)
13.0
Number of peaks
194 (qval < 1E-05)

Base call quality data from DBCLS SRA