Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Cardiovascular

Cell type

Circle of Willis

MeSH Description

A polygonal anastomosis at the base of the brain formed by the internal carotid (CAROTID ARTERY, INTERNAL), proximal parts of the anterior, middle, and posterior cerebral arteries (ANTERIOR CEREBRAL ARTERY; MIDDLE CEREBRAL ARTERY; POSTERIOR CEREBRAL ARTERY), the anterior communicating artery and the posterior communicating arteries.

Sample

source_name

Circle of Willis blood vessel

tissue

Circle of Willis

sample id

CoW4

gender

male

age

67

Cause of death

Aortic aneurysm

postmortem delay

9:00 hours

chip antibody

H3K4me1 (Abcam, ab8895)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Frozen in liquid nitrogen, Crosslinked, Lysed and Immunoprecipitated. Illumina TruSeq DNA library protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

32513657

Reads aligned (%)

96.9

Duplicates removed (%)

3.8

Number of peaks

1030 (qval < 1E-05)

hg19

Number of total reads

32513657

Reads aligned (%)

96.1

Duplicates removed (%)

4.9

Number of peaks

1173 (qval < 1E-05)