Homemade (ref. Morey et al., Cell Stem Cell, 2015)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown to 80% confluence on 150cm plates and processed using the High Sensitivity ChIP-IT Kit (Active Motif cat #53040). Immunoprecipitated DNA was used to generate libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs cat# 7370) following the manufacturer's instructions.