Antigen

Antigen Class

TFs and others

Antigen

MYOD1

Cell type

Cell type Class

Muscle

Cell type

Rhabdomyosarcoma

MeSH Description

A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)

Sample

source_name

Rhabdomyosarcoma

tissue

Rhabdomyosarcoma

chip antibody

MyoD

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Primary myoblast samples were collected in growth medium at low density, and both primary myotubes and RD cells were collected after being cultured in differentiation medium (72 h for myotubes, 24 h for RD cells). Libraries were generated from immunoprecipitated DNA as outlined in the Illumina DNA Library Construction Kit protocol except that 21 rounds of PCR were performed for amplification, and DNA fragments ranging from 200-350 bp were isolated during size selection on agarose gel.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

14195789

Reads aligned (%)

94.1

Duplicates removed (%)

5.6

Number of peaks

32472 (qval < 1E-05)

hg19

Number of total reads

14195789

Reads aligned (%)

93.7

Duplicates removed (%)

6.1

Number of peaks

32501 (qval < 1E-05)