Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYOD1

Cell type

Cell type Class
Muscle
Cell type
Myoblasts
MeSH Description
Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).

Attributes by original data submitter

Sample

source_name
human myoblast
tissue
myoblast
chip antibody
MyoD

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Primary myoblast samples were collected in growth medium at low density, and both primary myotubes and RD cells were collected after being cultured in differentiation medium (72 h for myotubes, 24 h for RD cells). Libraries were generated from immunoprecipitated DNA as outlined in the Illumina DNA Library Construction Kit protocol except that 21 rounds of PCR were performed for amplification, and DNA fragments ranging from 200-350 bp were isolated during size selection on agarose gel.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
23741977
Reads aligned (%)
64.2
Duplicates removed (%)
20.8
Number of peaks
26891 (qval < 1E-05)

hg38

Number of total reads
23741977
Reads aligned (%)
65.4
Duplicates removed (%)
19.8
Number of peaks
26867 (qval < 1E-05)

Base call quality data from DBCLS SRA