Antigen

Antigen Class

TFs and others

Antigen

MYOD1

Cell type

Cell type Class

Muscle

Cell type

Myoblasts

MeSH Description

Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).

Sample

source_name

human myoblast

tissue

myoblast

chip antibody

MyoD

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Primary myoblast samples were collected in growth medium at low density, and both primary myotubes and RD cells were collected after being cultured in differentiation medium (72 h for myotubes, 24 h for RD cells). Libraries were generated from immunoprecipitated DNA as outlined in the Illumina DNA Library Construction Kit protocol except that 21 rounds of PCR were performed for amplification, and DNA fragments ranging from 200-350 bp were isolated during size selection on agarose gel.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

23741977

Reads aligned (%)

81.5

Duplicates removed (%)

19.8

Number of peaks

26867 (qval < 1E-05)

hg19

Number of total reads

23741977

Reads aligned (%)

81.1

Duplicates removed (%)

20.8

Number of peaks

26891 (qval < 1E-05)