Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gata2

Cell type

Cell type Class
Blood
Cell type
FDCPmix
NA
NA

Attributes by original data submitter

Sample

source_name
FDCPmix
cell line
FDCPmix
Stage
5 days neutrophil differentiation
chip antibody
Gata2
chip antibody vendor
santa cruz biotechnology

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
FDCPmix cells were cross-linked with 1% formaldehyde and sonicated so that the bulk of the chromatin was between 100-500 bp. ChIP was essentially performed by standard procedures Libraries were made using the ChIP-Seq sample preparation kit (cat# IP-102-1001, Illumina, San Diego, CA, USA) as per manufacture’s instruction.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
19783805
Reads aligned (%)
97.5
Duplicates removed (%)
10.9
Number of peaks
2537 (qval < 1E-05)

mm9

Number of total reads
19783805
Reads aligned (%)
97.3
Duplicates removed (%)
11.0
Number of peaks
2801 (qval < 1E-05)

Base call quality data from DBCLS SRA