Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
HPC-7
NA
NA

Attributes by original data submitter

Sample

source_name
Cell culture passage 3
tissue
haematopoeitic progenitor cell line (HPC7)
genotype
Wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The library was completed and amplified through a PCR following the DeepSeq using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina default instructions (NEB #E7645). ChIP libraries were sequenced using an Illumina HiSeq 3000.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

mm10

Number of total reads
75967987
Reads aligned (%)
91.4
Duplicates removed (%)
12.8
Number of peaks
923 (qval < 1E-05)

mm9

Number of total reads
75967987
Reads aligned (%)
91.2
Duplicates removed (%)
13.2
Number of peaks
915 (qval < 1E-05)

Base call quality data from DBCLS SRA