Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

HPC-7

NA

NA

Sample

source_name

Cell culture passage 3

tissue

haematopoeitic progenitor cell line (HPC7)

genotype

Wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The library was completed and amplified through a PCR following the DeepSeq using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina default instructions (NEB #E7645). ChIP libraries were sequenced using an Illumina HiSeq 3000.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

47245669

Reads aligned (%)

97.1

Duplicates removed (%)

26.3

Number of peaks

256 (qval < 1E-05)

mm9

Number of total reads

47245669

Reads aligned (%)

97.0

Duplicates removed (%)

26.6

Number of peaks

244 (qval < 1E-05)