GSM2861544: KELLY DMSO CDK9; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CDK9
Cell type
Cell type Class
Neural
Cell type
KELLY
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
Chromatin IP against CDK9 in DMSO KELLY
cell line
KELLY
cell type
Neuroblastoma
input
KELLY_DMSO_WCE
treatment
0.1% DMSO for 3HR
growth protocol
10% FBS, DMEM
barcode
TGGTCACA
antibody
CDK9-H169
antibody maker
santa-cruz
antibody catalog number
sc-8338
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Tn5 enzyme and amplified using 2x NEBNext HiFi master mix. ChIPmentation (Schmidl et a., 2015; PMC4589892)