GSM2861542: KELLY CYC065 WCE; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Neural
Cell type
KELLY
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
Input for CYC065 KELLY
cell line
KELLY
cell type
Neuroblastoma
input
NA
treatment
1uM CYC065 for 3HR
growth protocol
10% FBS, DMEM
barcode
GTGTGGTG
antibody
NA
antibody maker
NA
antibody catalog number
NA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Tn5 enzyme and amplified using 2x NEBNext HiFi master mix. ChIPmentation (Schmidl et a., 2015; PMC4589892)