Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV-411
cell line
MV-411
cell type
leukemia cell line
treatment
none
chip antibody
H3K27ac Rabbit polyclonal antibody (Abcam, #4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer's recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
19956941
Reads aligned (%)
98.5
Duplicates removed (%)
7.1
Number of peaks
13527 (qval < 1E-05)

hg19

Number of total reads
19956941
Reads aligned (%)
97.8
Duplicates removed (%)
8.1
Number of peaks
13524 (qval < 1E-05)

Base call quality data from DBCLS SRA