Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Embryo

Cell type

6-8h embryos

NA

NA

Sample

source_name

Nuclei isolated from whole embryos ('WE')

developmental stage

6 to 8 hours after egg laying

tissue

whole embryo

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

Transgenic Drosophila melanogaster carrying a mesodermally expressed tagged histone marker (Bonn, S., Zinzen, R.P., Perez-Gonzalez, A., Riddell, A., Gavin, A.C., and Furlong, E.E. (2012). Cell type-specific chromatin immunoprecipitation from multicellula For DNase digestion, nuclei were resuspended in R-buffer (7.5mM Tris pH8, 45mM NaCl, 30mM KCl, 6mM MgCl2, 1mM CaCl2). Nuclei (4-20x10e6 per digest) were digested in 500uL R-buffer containing 2-20 units of DNaseI depending on nuclei number (where possible

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

53150262

Reads aligned (%)

90.9

Duplicates removed (%)

38.6

Number of peaks

13315 (qval < 1E-05)

dm3

Number of total reads

53150262

Reads aligned (%)

91.3

Duplicates removed (%)

30.2

Number of peaks

14965 (qval < 1E-05)