Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
S2 cells
RNAi treatment
CLAMP
chip antibody
N/A

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross linked in 1% formaldehyde and quenched with glycine prior to incubation in lysis buffer, and sonicated six times with 5 min cycles of 30s on/off o a Bioruptor sonicator. Samples were immunoprecipitated with either GAF, CLAMP, or MSL3 antibodies, and DNA clean-up was performed including RNase and Proteinase-K digestion steps. Libraries were prepared using the NEBNext Ultra II DNA library prep kit

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
6525567
Reads aligned (%)
89.9
Duplicates removed (%)
53.5
Number of peaks
2607 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA