GSM2860409: GAF RNAi CLAMP IP Replicate 2; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Clamp
Cell type
Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage
Attributes by original data submitter
Sample
source_name
S2 cells
RNAi treatment
GAF
chip antibody
rabbit anti-CLAMP
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross linked in 1% formaldehyde and quenched with glycine prior to incubation in lysis buffer, and sonicated six times with 5 min cycles of 30s on/off o a Bioruptor sonicator. Samples were immunoprecipitated with either GAF, CLAMP, or MSL3 antibodies, and DNA clean-up was performed including RNase and Proteinase-K digestion steps. Libraries were prepared using the NEBNext Ultra II DNA library prep kit