Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adipocyte
Cell type
Brown preadipocytes
NA
NA

Attributes by original data submitter

Sample

source_name
mouse brown preadipocytes, WT, input
strain/background
C57BL/6J
genotype/variation
MLL3-/-
age
E18.5 embryos
cell type
immortalized brown preadipocytes
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and MLL4-DNA complexes were isolated with homemade MLL4 antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
43775042
Reads aligned (%)
82.4
Duplicates removed (%)
12.8
Number of peaks
2034 (qval < 1E-05)

mm9

Number of total reads
43775042
Reads aligned (%)
82.2
Duplicates removed (%)
12.8
Number of peaks
1829 (qval < 1E-05)

Base call quality data from DBCLS SRA