Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FLI1

Cell type

Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
cell line
cell line
A673
chip antibody
FLI1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from formaldehyde-fixed cells (2-5 x 10^6 cells per epitope) was fragmented to a size range of 200 - 700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with the indicated antibodies and Protein G-Dynabeads (Life Technologies). Immunoprecipitated DNA was extracted with AMP Pure beads(Beckman Coulter) after crosslink reversal, RNAse A and Proteinase K treatment. ChIP DNA samples were used to generate Illumina sequencing libraries following standard protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
22401668
Reads aligned (%)
96.2
Duplicates removed (%)
23.4
Number of peaks
18171 (qval < 1E-05)

hg38

Number of total reads
22401668
Reads aligned (%)
97.2
Duplicates removed (%)
22.3
Number of peaks
18267 (qval < 1E-05)

Base call quality data from DBCLS SRA