Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Bone

Cell type

A-673

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

source_name

cell line

cell line

A673

chip antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin from formaldehyde-fixed cells (2-5 x 10^6 cells per epitope) was fragmented to a size range of 200 - 700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with the indicated antibodies and Protein G-Dynabeads (Life Technologies). Immunoprecipitated DNA was extracted with AMP Pure beads(Beckman Coulter) after crosslink reversal, RNAse A and Proteinase K treatment. ChIP DNA samples were used to generate Illumina sequencing libraries following standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

25924187

Reads aligned (%)

98.5

Duplicates removed (%)

13.4

Number of peaks

38866 (qval < 1E-05)

hg19

Number of total reads

25924187

Reads aligned (%)

98.3

Duplicates removed (%)

13.6

Number of peaks

38766 (qval < 1E-05)