Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
tsu

Cell type

Cell type Class
Cell line
Cell type
S2R+
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
ChIP Seq of HA tagged Y14
cell line
S2R+
cell type
Embryonic
chip antibody
F-7 (anti-HA), Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol NebNext UltraII

Sequencing Platform

instrument_model
NextSeq 500

dm3

Number of total reads
20311803
Reads aligned (%)
171.6
Duplicates removed (%)
9.9
Number of peaks
3886 (qval < 1E-05)

dm6

Number of total reads
20311803
Reads aligned (%)
169.2
Duplicates removed (%)
10.5
Number of peaks
3385 (qval < 1E-05)

Base call quality data from DBCLS SRA