Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Blood
Cell type
OCI-LY-10
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma B-cell

Attributes by original data submitter

Sample

source_name
ABC DLBCL cell line (LY10)
cell type
OCI-LY10
treatment
AZD1480(JAK inhibitor) for 4hrs
antibody
STAT3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments are followed by Rui et al., 2016, PNAS 113(46): E7260-E7267; Total mRNA was extracted by Rneasy plus mini kit (Qiagen) following the manufacturer's instructions. ChIP library was construbuted by using the Ovation Ultralow Library Kit (NuGen, Part No. 0330); cDNA library was construbuted by using Illumina Truseq RNA Library Prep Kit (RS-122-2002).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
121047789
Reads aligned (%)
76.1
Duplicates removed (%)
22.0
Number of peaks
1499 (qval < 1E-05)

hg38

Number of total reads
121047789
Reads aligned (%)
77.7
Duplicates removed (%)
20.9
Number of peaks
1439 (qval < 1E-05)

Base call quality data from DBCLS SRA