Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Blood
Cell type
TMD8
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell

Attributes by original data submitter

Sample

source_name
ABC DLBCL cell line (TMD8)
cell type
TMD8
treatment
AZD1480(JAK inhibitor) for 4hrs
antibody
STAT3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments are followed by Rui et al., 2016, PNAS 113(46): E7260-E7267; Total mRNA was extracted by Rneasy plus mini kit (Qiagen) following the manufacturer's instructions. ChIP library was construbuted by using the Ovation Ultralow Library Kit (NuGen, Part No. 0330); cDNA library was construbuted by using Illumina Truseq RNA Library Prep Kit (RS-122-2002).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
119720803
Reads aligned (%)
68.8
Duplicates removed (%)
29.6
Number of peaks
1442 (qval < 1E-05)

hg38

Number of total reads
119720803
Reads aligned (%)
70.3
Duplicates removed (%)
28.5
Number of peaks
1495 (qval < 1E-05)

Base call quality data from DBCLS SRA