Cells were cultured for 7 days with IL-2, fixed and frozen at -80°C.
strain
ICR
chip antibody
Monoclonal anti-H3K4me1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cultured NK cells were fixed in 1% formaldehyde and sonicated to yield DNA fragments of ~300bp. For immunoprecipitation, 48ul of anti-Runx3 Ab, anti-H3K4me1 or NIS were added to 12 mL of diluted, fragmented chromatin. DNA was purified using QIAquick spin columns (QIAGEN). For ChIP-seq analysis, ChIP-seq libraries were prepared using the ChIP-Seq sample preparation kit (IP-102-1001, illumina) according to the manufactures instructions. Illumina sequencing was performed using Illumina genome analyzer IIx according to the manufactures instructions by loading 15 pmoles of denatured library template onto separate lanes of a flow cell. Sequencing short reads (40 bp) were aligned to the mouse genome (mmp9) using the ELAND program (Illumina).