A front limb of a quadruped. (The Random House College Dictionary, 1980)
Attributes by original data submitter
Sample
source_name
E10.5 forelimb buds
chip antibody
Twist1 (Sigma, T6451, 5 μg)
tissue
E10.5 limb bud
strain
FVB/N
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP experiments, ECCs, limb buds, and PNST cells were cross-linked and isolated according to manufacturer’s protocols (Magnify ChIP kit, Invitrogen). Crosslinked DNA complexes were sheared by sonication (Virsonic 60, Virtis, output of 5) 6 times for 5 seconds with a 2-minute refractory period on ice. Samples were sheared to a size of 150-250 basepairs (bp). ChIP was then performed according to the manufacturer’s protocol (Magnify ChIP Kit, Invitrogen). A single read genomic DNA library was generated using an IntegenX robot (Apollo 324) and PrepX DNA library kit (IntegenX 400040) was utilized according to manufacturer's protocols on mouse genomic DNA.