Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Neural
Cell type
Prefrontal Cortex
MeSH Description
The rostral part of the frontal lobe, bounded by the inferior precentral fissure in humans, which receives projection fibers from the MEDIODORSAL NUCLEUS OF THE THALAMUS. The prefrontal cortex receives afferent fibers from numerous structures of the DIENCEPHALON; MESENCEPHALON; and LIMBIC SYSTEM as well as cortical afferents of visual, auditory, and somatic origin.

Attributes by original data submitter

Sample

source_name
prefrontal cortex
tissue
prefrontal cortex
age
Medium age 46 year old
chip antibody
H3K27ac (Abcam, Cat. # ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissues were homogenize and crosslinked, 10 m~1mg lysis buffer. Samples were sonicated for 40 min. ChIP libraries were constructed using Illumina standard protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
28975375
Reads aligned (%)
99.4
Duplicates removed (%)
3.1
Number of peaks
21555 (qval < 1E-05)

hg19

Number of total reads
28975375
Reads aligned (%)
99.0
Duplicates removed (%)
3.3
Number of peaks
21522 (qval < 1E-05)

Base call quality data from DBCLS SRA