Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETV1

Cell type

Cell type Class
Prostate
Cell type
MDA PCa 2b
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-PCa2b-ETV1
cell line
MDA-PCa2b

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
cells were crosslinked for 15 min in 1% paraformaldehyde, washed and lysed. Chromatin was sheared using Bioruptor (Diagenode) to fragments of approximately 150 base pairs and was incubated with anti-rabbit IgG Dynabeads (Invitrogen) pre-conjugated with primary antibody,  ChIP libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27933652
Reads aligned (%)
49.4
Duplicates removed (%)
5.7
Number of peaks
16299 (qval < 1E-05)

hg38

Number of total reads
27933652
Reads aligned (%)
50.5
Duplicates removed (%)
4.4
Number of peaks
16279 (qval < 1E-05)

Base call quality data from DBCLS SRA