GSM2844032: MDA-PCa2b-ETV1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ETV1
Cell type
Cell type Class
Prostate
Cell type
MDA PCa 2b
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-PCa2b-ETV1
cell line
MDA-PCa2b
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
cells were crosslinked for 15 min in 1% paraformaldehyde, washed and lysed. Chromatin was sheared using Bioruptor (Diagenode) to fragments of approximately 150 base pairs and was incubated with anti-rabbit IgG Dynabeads (Invitrogen) pre-conjugated with primary antibody, ChIP libraries were prepared for sequencing using standard Illumina protocols