Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Digestive tract

Cell type

GIST-T1

NA

NA

Sample

source_name

GIST-T1-input-1

cell line

GIST-T1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

cells were crosslinked for 15 min in 1% paraformaldehyde, washed and lysed. Chromatin was sheared using Bioruptor (Diagenode) to fragments of approximately 150 base pairs and was incubated with anti-rabbit IgG Dynabeads (Invitrogen) pre-conjugated with primary antibody,  ChIP libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

18156347

Reads aligned (%)

81.7

Duplicates removed (%)

2.7

Number of peaks

545 (qval < 1E-05)

hg19

Number of total reads

18156347

Reads aligned (%)

81.1

Duplicates removed (%)

3.8

Number of peaks

605 (qval < 1E-05)