Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOSL2

Cell type

Cell type Class
Epidermis
Cell type
SK-MEL-147
NA
NA

Attributes by original data submitter

Sample

source_name
Cell culture
cell line
SKmel147
passage
N/A
antibodies
FOSL2 (Santa Cruz SC-604)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For FOSL2 and TEAD4, 10x10^6 cells, were crosslinked with 0.25M DSG for 45min, followed by 1% PFA crosslinking for 10 min at RT. ChIP was performed essentially as previously described (Tian et al., Two-step Crosslinking for Analysis of Protein-chroamtin Interactions. Methods Mol Biol, 2012. 809:105-120. Roe, J.S., et al., BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Mol Cell, 2015. 58(6): p. 1028-39). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
35439912
Reads aligned (%)
73.3
Duplicates removed (%)
17.1
Number of peaks
57819 (qval < 1E-05)

hg38

Number of total reads
35439912
Reads aligned (%)
75.0
Duplicates removed (%)
15.7
Number of peaks
58217 (qval < 1E-05)

Base call quality data from DBCLS SRA