Antigen

Antigen Class

TFs and others

Antigen

SOX2

Cell type

Cell type Class

Digestive tract

Cell type

TE-5

Primary Tissue

Esophagus

Tissue Diagnosis

Carcinoma Squamous Cell

Sample

source_name

TE5

cell line

TE5

cell type

Esophageal squamous carcinoma cells

chip antibody

SOX2 [AF2018 (R&D System), ab59776 (Abcam) or 3579S (Cell Signaling Technology)]

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Standard Illumina library preparation protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

22349786

Reads aligned (%)

94.0

Duplicates removed (%)

5.3

Number of peaks

684 (qval < 1E-05)

hg19

Number of total reads

22349786

Reads aligned (%)

93.2

Duplicates removed (%)

6.5

Number of peaks

874 (qval < 1E-05)