Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Bone marrow
cell type
HSCe (CD34+, CD38-,Lineage-)
donor age
66
donor sex
female
antibody
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Micro-ChIPseq was performed using the True MicroChIP (Diagenode, #C01010130) kit and 0.5 ug H3 (Abcam ab10799, lot #GR275925-1) per immunoprecipitation ChIP-seq libraries were prepared using the V1 MicroPlex Library Preparation kit (Diagenode, #C05010011). For the PCR amplification, a total of 16 amplification cycles was used. Libraries were purified using a 1:1 Ampure bead cleanup and eluted in 16 uL of Tris-HCl ph 8.0. Libraries were multiplexed and sequenced using a NextSeq 500 with 75bp single end sequencing.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
46142878
Reads aligned (%)
95.0
Duplicates removed (%)
21.5
Number of peaks
1353 (qval < 1E-05)

hg19

Number of total reads
46142878
Reads aligned (%)
94.1
Duplicates removed (%)
22.9
Number of peaks
1142 (qval < 1E-05)

Base call quality data from DBCLS SRA