Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFP

Cell type

Cell type Class
Embryo
Cell type
Late embryo
NA
NA

Attributes by original data submitter

Sample

source_name
late embryos_CFP-1::GFP ChIP seq
strain
JA1597
tissue
whole embryo
Stage
Late embryo
chip antibody
anti-GFP
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were made using the Truseq DNA sample prep kit (Illumina). 1-10ng of ChIP or 10ng of input are blunted (T4 polymerase), an A-overhang is added (Klenow), DNA is ligated to adaptors, amplified by PCR, and the library size-selected to a 250-400 bp range using Agencourt AMPure XP beads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
13765284
Reads aligned (%)
87.3
Duplicates removed (%)
22.0
Number of peaks
5266 (qval < 1E-05)

ce10

Number of total reads
13765284
Reads aligned (%)
87.3
Duplicates removed (%)
22.0
Number of peaks
5269 (qval < 1E-05)

Base call quality data from DBCLS SRA