Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4ac

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HUVEC_acetylated H4_minus
cell type
human umbilical vein endothelial cells
stimulation
none
chip antibody
H4ac (06-866; Upstate)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were extracted from fixed cells with paraformaldehyde and sonicated protein-DNA complexes were isolated with antibody. Libraries were prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) according to the instruction manuals.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
24585265
Reads aligned (%)
83.4
Duplicates removed (%)
44.5
Number of peaks
632 (qval < 1E-05)

hg19

Number of total reads
24585265
Reads aligned (%)
83.0
Duplicates removed (%)
45.3
Number of peaks
657 (qval < 1E-05)

Base call quality data from DBCLS SRA