Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFATC1

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HUVEC_NFATc1_minus
cell type
human umbilical vein endothelial cells
stimulation
none
chip antibody
NFATc1 (generated by our laboratories)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were extracted from fixed cells with paraformaldehyde and sonicated protein-DNA complexes were isolated with antibody. Libraries were prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) according to the instruction manuals.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
54080831
Reads aligned (%)
36.4
Duplicates removed (%)
53.7
Number of peaks
9009 (qval < 1E-05)

hg38

Number of total reads
54080831
Reads aligned (%)
38.1
Duplicates removed (%)
52.1
Number of peaks
9059 (qval < 1E-05)

Base call quality data from DBCLS SRA